The Mouse MBP (Myelin Basic Protein) ELISA Kit is a reliable and sensitive assay designed for the detection of MBP levels in mouse serum, plasma, and cell culture supernatants. This kit offers high specificity and sensitivity, ensuring accurate and reproducible results for a variety of research applications.Myelin Basic Protein (MBP) is a key component of the myelin sheath in the nervous system and plays a crucial role in the proper functioning of nerve cells. Changes in MBP levels have been associated with various neurological disorders such as multiple sclerosis and other demyelinating diseases, making it an important biomarker for studying these conditions and potential therapeutic interventions. Overall, the Mouse MBP ELISA Kit provides researchers with a valuable tool for studying the role of MBP in neurodegenerative disorders and other related conditions, leading to a better understanding of disease mechanisms and potential treatment options.
Product Name:
Mouse MBP (Myelin Basic Protein) ELISA Kit
SKU:
MOES01280
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.1 ng/mL
Detection range:
0.16-10 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
