Mouse MCP-1 (Monocyte Chemotactic Protein 1) CLIA Kit
The Mouse MCP-1 (Monocyte Chemotactic Protein-1) CLIA Kit is a highly reliable and sensitive assay designed for the accurate measurement of MCP-1 levels in mouse serum, plasma, and cell culture supernatants. This kit provides precise and reproducible results, making it ideal for various research applications.MCP-1 is a key chemokine involved in the recruitment and activation of monocytes, playing a crucial role in inflammation and immune response. Elevated levels of MCP-1 have been implicated in various inflammatory diseases, making it a valuable biomarker for studying these conditions and identifying potential therapeutic targets.
With its high specificity and ease of use, the Mouse MCP-1 CLIA Kit is a valuable tool for researchers looking to investigate the role of MCP-1 in inflammation, immune response, and related diseases in mouse models. Get accurate and reliable results with this innovative assay kit from Assay Genie.
Product Name:
Mouse MCP-1 (Monocyte Chemotactic Protein 1) CLIA Kit
SKU:
MOES00006
Target:
Mouse MCP-1 (Monocyte Chemotactic Protein 1)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse MCP-1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse MCP-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse MCP-1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse MCP-1. You can calculate the concentration of Mouse MCP-1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
90-104
91-105
102-118
Average (%)
98
98
110
1:4
Range (%)
96-109
89-105
87-100
Average (%)
102
96
92
1:8
Range (%)
86-98
94-108
86-99
Average (%)
91
100
92
1:16
Range (%)
99-113
98-109
100-114
Average (%)
107
104
105
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-104
97
EDTA plasma (n=5)
93-107
100
Cell culture media (n=5)
101-116
108
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
23.42
46.95
200.65
22.29
50.44
219.52
Standard deviation
2.46
4.0
13.0
2.49
4.23
22.92
C V (%)
10.5
8.52
6.48
11.17
8.39
10.44
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse MCP-1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse MCP-1 in samples. No significant cross-reactivity or interference between Mouse MCP-1 and analogues was observed.
