Mouse MCP-1 (Monocyte Chemotactic Protein 1) ELISA Kit
The Mouse MCP-1 (Monocyte Chemotactic Protein 1) ELISA Kit is specifically designed for the quantitative measurement of MCP-1 levels in various biological samples obtained from mouse models.
MCP-1 is a crucial chemokine involved in immune responses, inflammation, and the recruitment of monocytes to sites of injury or infection, making it a key player in the regulation of inflammatory processes and immune cell functions. Assay Genie's Mouse MCP-1 ELISA Kit offers high sensitivity and specificity, allowing for accurate and reproducible detection of MCP-1 levels. With stringent quality control measures and validated protocols, this kit ensures robust performance and ease of use, making it an excellent choice for researchers investigating the roles of MCP-1 in inflammation, immune responses, and related diseases. Trust Assay Genie's Mouse MCP-1 ELISA Kit for precise quantification of MCP-1 levels in your research endeavors.
Product Name:
Mouse MCP-1 (Monocyte Chemotactic Protein 1) ELISA Kit
SKU:
AEES00199
Target:
Mouse MCP-1 (Monocyte Chemotactic Protein 1)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse MCP-1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse MCP-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse MCP-1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse MCP-1. You can calculate the concentration of Mouse MCP-1 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
82-98
84-98
83-97
Average (%)
90
92
90
1:4
Range (%)
84-96
91-101
93-102
Average (%)
92
98
98
1:8
Range (%)
92-107
94-104
86-96
Average (%)
100
98
91
1:16
Range (%)
93-108
84-94
95-102
Average (%)
101
90
98
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-104
100
EDTA plasma (n=5)
87-99
92
Cell culture media (n=5)
90-100
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
94.05
210.98
994.91
88.91
244.77
840.43
Standard deviation
5.14
10.06
47.86
4.82
12.09
45.97
C V (%)
5.47
4.77
4.81
5.42
4.94
5.47
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse MCP-1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse MCP-1 in samples. No significant cross-reactivity or interference between Mouse MCP-1 and analogues was observed.
