Mouse MIP-1 alpha (Macrophage Inflammatory Protein 1 Alpha) CLIA Kit
The Mouse MIP-1 Alpha (Macrophage Inflammatory Protein-1 Alpha) CLIA Kit is a comprehensive tool designed for the quantitative measurement of MIP-1 Alpha levels in mouse serum, plasma, and tissue homogenates. This kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results for a variety of research applications.MIP-1 Alpha is a key chemokine involved in the recruitment and activation of immune cells, playing a critical role in inflammation, immune responses, and host defense mechanisms.
Its dysregulation has been implicated in various inflammatory diseases, making it a valuable biomarker for studying these conditions and potential therapeutic interventions.With its advanced technology and reliable performance, the Mouse MIP-1 Alpha CLIA Kit is an essential tool for researchers investigating the role of MIP-1 Alpha in immune responses, inflammation, and disease pathogenesis. Get accurate and precise results with this high-quality kit from AssayGenie.
Product Name:
Mouse MIP-1 alpha (Macrophage Inflammatory Protein 1 Alpha) CLIA Kit
SKU:
MOES00007
Target:
Mouse MIP-1 alpha (Macrophage Inflammatory Protein 1 Alpha)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse MIP-1α. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse MIP-1α and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse MIP-1α, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse MIP-1α. You can calculate the concentration of Mouse MIP-1α in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
91-106
94-111
93-107
Average (%)
97
101
98
1:4
Range (%)
86-99
100-114
99-113
Average (%)
91
108
105
1:8
Range (%)
88-102
95-112
91-101
Average (%)
94
103
96
1:16
Range (%)
98-115
84-96
98-116
Average (%)
107
90
106
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
100-117
107
EDTA plasma (n=5)
87-97
92
Cell culture media (n=5)
99-114
107
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
25.47
78.9
199.14
27.13
83.41
203.33
Standard deviation
2.4
8.62
14.36
2.98
7.45
15.58
C V (%)
9.42
10.93
7.21
10.98
8.93
7.66
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse MIP-1α concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse MIP-1α in samples. No significant cross-reactivity or interference between Mouse MIP-1α and analogues was observed.
