Mouse MIP-1 alpha (Macrophage Inflammatory Protein 1 Alpha) ELISA Kit
The Mouse MIP-1 alpha (Macrophage Inflammatory Protein-1 alpha) ELISA Kit is a reliable and accurate tool for detecting levels of MIP-1 alpha in mouse serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides consistent and reproducible results, making it perfect for a variety of research applications.MIP-1 alpha is a key chemokine involved in the inflammatory response, attracting and activating immune cells such as macrophages and T cells. It plays a crucial role in various biological processes, including immune response regulation and inflammation resolution.
Studying MIP-1 alpha levels can provide essential insights into inflammatory diseases, immune disorders, and other pathological conditions, offering valuable information for potential therapeutic developments.Overall, the Mouse MIP-1 alpha (Macrophage Inflammatory Protein-1 alpha) ELISA Kit is a valuable tool for researchers seeking to investigate the role of MIP-1 alpha in various biological processes and disease states, ultimately advancing our understanding of immune response mechanisms and potential treatment options.
Product Name:
Mouse MIP-1 alpha (Macrophage Inflammatory Protein 1 Alpha) ELISA Kit
SKU:
MOES00628
Target:
Mouse MIP-1 alpha (Macrophage Inflammatory Protein 1 Alpha)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse MIP-1α. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse MIP-1α and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse MIP-1α, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse MIP-1α. You can calculate the concentration of Mouse MIP-1α in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-110
93-108
88-101
Average (%)
102
99
94
1:4
Range (%)
98-113
83-95
96-112
Average (%)
104
88
103
1:8
Range (%)
96-111
81-94
92-108
Average (%)
103
87
100
1:16
Range (%)
98-112
85-98
91-109
Average (%)
105
90
99
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-99
92
EDTA plasma (n=5)
90-103
95
Cell culture media (n=5)
89-105
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
46.9
154.8
386.6
42.7
160.0
368.2
Standard deviation
2.4
8.0
13.9
2.9
9.4
19.9
C V (%)
5.12
5.17
3.6
6.79
5.88
5.4
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse MIP-1α concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse MIP-1α in samples. No significant cross-reactivity or interference between Mouse MIP-1α and analogues was observed.
