Mouse MIP-1 beta (Macrophage Inflammatory Protein 1 Beta) ELISA Kit
The Mouse MIP-1 beta (Macrophage Inflammatory Protein-1 beta) ELISA Kit is specifically designed for the accurate measurement of MIP-1 beta levels in mouse serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit delivers reliable and reproducible results, making it an essential tool for various research applications.MIP-1 beta is a key cytokine involved in the inflammatory response, playing a crucial role in immune cell recruitment and activation. Its dysregulation has been implicated in various inflammatory diseases and conditions, making it a valuable biomarker for studying and understanding these pathologies.This ELISA kit provides researchers with a powerful tool for quantifying MIP-1 beta levels in mouse samples, allowing for precise and accurate measurements that can aid in the investigation of inflammatory processes and potential therapeutic interventions.
Product Name:
Mouse MIP-1 beta (Macrophage Inflammatory Protein 1 Beta) ELISA Kit
SKU:
MOES00629
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
