The Mouse MMP-3 (Matrix Metalloproteinase-3) CLIA Kit is a comprehensive assay kit designed for the accurate measurement of MMP-3 levels in mouse serum, plasma, and tissue homogenates. This kit offers exceptional sensitivity and specificity, ensuring reliable and reproducible results for a variety of research applications.MMP-3, also known as stromelysin-1, is a key enzyme involved in extracellular matrix remodeling and tissue repair. It plays a critical role in various physiological and pathological processes, including tissue remodeling, inflammation, and cancer metastasis.
Understanding MMP-3 levels is essential for studying these conditions and developing potential therapeutic interventions.The Mouse MMP-3 CLIA Kit from AssayGenie provides researchers with a powerful tool to study the role of MMP-3 in disease progression and therapeutic response. With its high-quality reagents and easy-to-use protocol, this kit is an essential addition to any research laboratory focused on matrix metalloproteinase biology.
Product Name:
Mouse MMP-3 (Matrix Metalloproteinase 3) CLIA Kit
SKU:
MOES00333
Target:
Mouse MMP-3 (Matrix Metalloproteinase 3)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse MMP-3. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse MMP-3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse MMP-3, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse MMP-3. You can calculate the concentration of Mouse MMP-3 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-106
98-110
93-108
Average (%)
100
105
98
1:4
Range (%)
100-113
88-100
86-98
Average (%)
107
95
92
1:8
Range (%)
97-109
95-107
97-114
Average (%)
103
101
104
1:16
Range (%)
91-103
95-107
96-111
Average (%)
98
100
103
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-101
95
EDTA plasma (n=5)
98-115
106
Cell culture media (n=5)
93-104
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
107.08
246.09
778.31
117.54
256.97
828.72
Standard deviation
10.45
21.43
72.54
13.2
20.79
91.49
C V (%)
9.76
8.71
9.32
11.23
8.09
11.04
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse MMP-3 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse MMP-3 in samples. No significant cross-reactivity or interference between Mouse MMP-3 and analogues was observed.
