The Mouse MUC5B (Mucin 5 Subtype B) CLIA Kit is a powerful tool for the quantitative measurement of MUC5B levels in mouse serum, plasma, and tissue lysates. This kit offers high sensitivity and specificity, ensuring accurate and reliable results for a variety of research applications.MUC5B is a mucin protein that plays a critical role in mucin production and airway defense. Abnormalities in MUC5B have been linked to respiratory diseases such as asthma, COPD, and cystic fibrosis, making it a valuable biomarker for studying these conditions and developing potential therapeutic interventions.
With its easy-to-use format and precise measurements, the Mouse MUC5B CLIA Kit is an essential tool for researchers and scientists looking to explore the role of MUC5B in respiratory health and disease progression.
Product Name:
Mouse MUC5B (Mucin-5 Subtype B) CLIA Kit
SKU:
MOES00442
Target:
Mouse MUC5B (Mucin-5 Subtype B)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse MUC5B. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse MUC5B and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse MUC5B, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse MUC5B. You can calculate the concentration of Mouse MUC5B in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
91-106
88-102
92-109
Average (%)
98
94
100
1:4
Range (%)
88-103
84-96
103-118
Average (%)
94
91
110
1:8
Range (%)
95-109
88-98
98-115
Average (%)
101
93
106
1:16
Range (%)
97-111
97-113
95-108
Average (%)
104
103
101
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
97-112
103
EDTA plasma (n=5)
97-113
103
Cell culture media (n=5)
87-101
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
187.58
451.91
1482.49
183.21
440.24
1396.64
Standard deviation
16.36
48.54
141.58
17.5
50.94
159.78
C V (%)
8.72
10.74
9.55
9.55
11.57
11.44
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse MUC5B concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse MUC5B in samples. No significant cross-reactivity or interference between Mouse MUC5B and analogues was observed.
