Mouse NMNAT1 Recombinant Protein (RPPB2026)
- SKU:
- RPPB2026
- Product Type:
- Recombinant Protein
- Species:
- Mouse
- Uniprot:
- Q9EPA7
- Research Area:
- Enzymes
Description
| Product Name: | Mouse NMNAT1 Recombinant Protein |
| Product Code: | RPPB2026 |
| Size: | 5µg |
| Species: | Mouse |
| Target: | NMNAT1 |
| Synonyms: | Nicotinamide/nicotinic acid mononucleotide adenylyltransferase 1, NMNAT1, NMN/NaMN adenylyltransferase 1, Nicotinamide mononucleotide adenylyltransferase 1, NMN adenylyltransferase, Nicotinate-nucleotide adenylyltransferase 1, NaMN adenylyltransferase 1, D4Cole1e, Nmnat. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile filtered colourless solution. |
| Formulation: | NMNAT1 protein solution (1mg/ml) containing Phosphate Buffered Saline (pH7.4), 20% glycerol and 1mM EDTA. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks.Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 95.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGSMDSSKKT EVVLLACGSF NPITNMHLRL FELAKDYMHA TGKYSVIKGI ISPVGDAYKK KGLIPAHHRI IMAELATKNS HWVEVDTWES LQKEWVETVK VLRYHQEKLA TGSCSYPQSS PALEKPGRKR KWADQKQDSS PQKPQEPKPT GVPKVKLLCG ITNDISSTKI RRALRRGQSI RYLVPDLVQE YIEKHELYNT ESEGRNAGVT LAPLQRNAAE AKHNHSTL |
NMNAT1 enzyme is vital for NAD biosynthesis, catalyzing the condensation of nicotinamide mononucleotide (NMN) or nicotinic acid mononucleotide (NaMN) with the AMP moiety of ATP to form NAD or NaAD. NMNAT1 is widely expressed with high levels in skeletal muscle, heart, liver and kidney. This protein appears to have the ability to protect against axonal degeneration following mechanical or toxic insults.
NMNAT1 Mouse Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 308 amino acids (1-285 a.a) and having a molecular mass of 34.7kDa. NMNAT1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.�
| UniProt Protein Function: | NMNAT1: Catalyzes the formation of NAD(+) from nicotinamide mononucleotide (NMN) and ATP. Can also use the deamidated form; nicotinic acid mononucleotide (NaMN) as substrate with the same efficiency. Can use triazofurin monophosphate (TrMP) as substrate. Also catalyzes the reverse reaction, i.e. the pyrophosphorolytic cleavage of NAD(+). For the pyrophosphorolytic activity, prefers NAD(+) and NAAD as substrates and degrades NADH, nicotinic acid adenine dinucleotide phosphate (NHD) and nicotinamide guanine dinucleotide (NGD) less effectively. Fails to cleave phosphorylated dinucleotides NADP(+), NADPH and NAADP(+). Protects against axonal degeneration following mechanical or toxic insults. Belongs to the eukaryotic NMN adenylyltransferase family. |
| UniProt Protein Details: | Protein type:Cofactor and Vitamin Metabolism - nicotinate and nicotinamide; EC 2.7.7.1; EC 2.7.7.18; Transferase Chromosomal Location of Human Ortholog: 4|4 E2 Cellular Component: nuclear body; nucleoplasm; nucleus Molecular Function:identical protein binding; nicotinamide-nucleotide adenylyltransferase activity; nicotinate-nucleotide adenylyltransferase activity Biological Process: NAD biosynthetic process; response to wounding |
| UniProt Code: | Q9EPA7 |
| NCBI GenInfo Identifier: | 30580490 |
| NCBI Gene ID: | 66454 |
| NCBI Accession: | Q9EPA7.2 |
| UniProt Secondary Accession: | Q9EPA7,Q6B504, |
| UniProt Related Accession: | Q9EPA7 |
| Molecular Weight: | 32,355 Da |
| NCBI Full Name: | Nicotinamide/nicotinic acid mononucleotide adenylyltransferase 1 |
| NCBI Synonym Full Names: | nicotinamide nucleotide adenylyltransferase 1 |
| NCBI Official Symbol: | Nmnat1�� |
| NCBI Official Synonym Symbols: | nmnat; D4Cole1e; 2610529L11Rik; 5730441G13Rik�� |
| NCBI Protein Information: | nicotinamide/nicotinic acid mononucleotide adenylyltransferase 1 |
| UniProt Protein Name: | Nicotinamide/nicotinic acid mononucleotide adenylyltransferase 1 |
| UniProt Synonym Protein Names: | Nicotinamide mononucleotide adenylyltransferase 1; NMN adenylyltransferase 1; Nicotinate-nucleotide adenylyltransferase 1; NaMN adenylyltransferase 1 |
| UniProt Gene Name: | Nmnat1�� |
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