The Mouse OC (BGP Osteocalcin) CLIA Kit is a powerful tool for measuring levels of Osteocalcin in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, providing accurate and consistent results for a variety of research applications.Osteocalcin is a key protein involved in bone formation and regulation, playing a crucial role in maintaining bone health and metabolism.
Changes in Osteocalcin levels have been linked to conditions such as osteoporosis, diabetes, and cardiovascular diseases, making it a valuable biomarker for studying these diseases and developing therapeutic interventions.With its user-friendly format and reliable performance, the Mouse OC CLIA Kit from Assay Genie is an essential tool for researchers investigating the role of Osteocalcin in health and disease.
Product Name:
Mouse OC/BGP (Osteocalcin) CLIA Kit
SKU:
MOES00463
Target:
Mouse OC/BGP (Osteocalcin)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
46.88 pg/mL
Detection range:
78.13-5000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse OC/BGP. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse OC/BGP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse OC/BGP, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse OC/BGP. You can calculate the concentration of Mouse OC/BGP in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-108
89-100
101-120
Average (%)
101
94
109
1:4
Range (%)
97-115
92-106
103-119
Average (%)
105
98
110
1:8
Range (%)
91-102
86-99
93-109
Average (%)
96
91
100
1:16
Range (%)
101-117
93-105
90-105
Average (%)
107
98
97
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-105
100
EDTA plasma (n=5)
88-102
96
Cell culture media (n=5)
83-95
90
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
251.98
741.72
1967.18
250.95
681.11
1925.51
Standard deviation
31.67
59.78
210.09
29.54
70.49
220.28
C V (%)
12.57
8.06
10.68
11.77
10.35
11.44
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse OC/BGP concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse OC/BGP in samples. No significant cross-reactivity or interference between Mouse OC/BGP and analogues was observed.
