The Mouse Pro-MMP-9 ELISA Kit is expertly designed to facilitate the precise quantitative analysis of Pro-Matrix Metalloproteinase-9 (Pro-MMP-9) levels in a variety of biological samples. Pro-MMP-9, also known as Progelatinase B, is an essential enzyme involved in extracellular matrix remodeling and tissue repair processes.
This proenzyme plays a critical role in activating active MMP-9, which contributes to various physiological and pathological conditions, including tissue regeneration, metastasis, and inflammation. Accurate measurement of Pro-MMP-9 levels is crucial for understanding its functions in regulating the extracellular matrix dynamics and its implications in tissue remodeling and cell migration processes. By quantifying Pro-MMP-9 expression, researchers can gain insights into its involvement in various cellular activities and disease progression, particularly in conditions associated with tissue remodeling and matrix degradation. The Mouse Pro-MMP-9 ELISA Kit from Assay Genie offers exceptional sensitivity and specificity, ensuring dependable and reproducible results. With stringent quality control measures in place, this kit delivers robust performance and user-friendly protocols, making it an ideal choice for research endeavors focused on investigating the roles of Pro-Matrix Metalloproteinase-9 in biological processes and disease pathogenesis.
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse Pro-MMP-9. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse Pro-MMP-9 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse Pro-MMP-9, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse Pro-MMP-9. You can calculate the concentration of Mouse Pro-MMP-9 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
99-114
88-104
98-114
Average (%)
106
95
105
1:4
Range (%)
97-111
88-101
99-115
Average (%)
105
94
105
1:8
Range (%)
98-116
88-100
94-108
Average (%)
106
94
101
1:16
Range (%)
100-115
87-100
99-114
Average (%)
107
92
104
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
85-100
91
EDTA plasma (n=5)
91-105
96
Cell culture media (n=5)
89-101
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
253.11
435.12
1790.24
258.34
407.4
1655.43
Standard deviation
17.26
22.54
88.08
16.82
17.88
69.53
C V (%)
6.82
5.18
4.92
6.51
4.39
4.2
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse Pro-MMP-9 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse Pro-MMP-9 in samples. No significant cross-reactivity or interference between Mouse Pro-MMP-9 and analogues was observed.
