The Mouse PSA (Prostate Specific Antigen) ELISA Kit is specifically designed for the precise measurement of PSA levels in mouse serum, plasma, and cell culture supernatants. This kit provides exceptional sensitivity and specificity, guaranteeing accurate and consistent results, making it perfect for various research purposes.PSA is a vital protein linked to prostate health, often used as a biomarker for prostate cancer diagnosis and monitoring.
By measuring PSA levels, researchers can gain valuable insights into prostate-related conditions, facilitating the development of effective treatments and therapies.This ELISA kit offers a reliable and efficient way to analyze PSA levels in mouse samples, providing researchers with a powerful tool to advance their studies in prostate health and related research areas.
Product Name:
Mouse PSA (Prostate Specific Antigen) ELISA Kit
SKU:
MOES01402
Target:
Mouse PSA (Prostate Specific Antigen)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse PSA. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse PSA and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse PSA, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse PSA. You can calculate the concentration of Mouse PSA in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
91-104
93-106
96-110
Average (%)
97
100
104
1:4
Range (%)
91-104
80-94
86-101
Average (%)
97
87
93
1:8
Range (%)
91-101
87-98
81-95
Average (%)
96
92
88
1:16
Range (%)
88-101
83-97
83-95
Average (%)
94
90
88
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-106
98
EDTA plasma (n=5)
86-98
92
Cell culture media (n=5)
96-112
102
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.53
1.06
4.38
0.57
1.04
4.58
Standard deviation
0.03
0.05
0.14
0.04
0.05
0.21
C V (%)
5.66
4.72
3.2
7.02
4.81
4.59
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse PSA concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse PSA in samples. No significant cross-reactivity or interference between Mouse PSA and analogues was observed.
