Mouse RBP4 (Retinol Binding Protein 4, Plasma) CLIA Kit
The Mouse RBP4 (Retinol Binding Protein 4) Plasma CLIA Kit is specifically designed for the quantitative detection of RBP4 levels in mouse plasma samples. This kit offers high sensitivity and accuracy, ensuring precise and reliable results for research purposes.RBP4 is a key protein involved in the transport of retinol (vitamin A) in the blood, playing a crucial role in metabolic functions and insulin resistance. Dysregulation of RBP4 levels has been linked to various metabolic disorders and diseases, making it a valuable biomarker for studying these conditions and potential therapeutic interventions.
With its advanced technology and proven performance, the Mouse RBP4 Plasma CLIA Kit is a valuable tool for researchers investigating the role of RBP4 in metabolic pathways, insulin sensitivity, and related diseases in mouse models.
Product Name:
Mouse RBP4 (Retinol Binding Protein 4, Plasma) CLIA Kit
SKU:
MOES00517
Target:
Mouse RBP4 (Retinol Binding Protein 4, Plasma)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse RBP4. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse RBP4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse RBP4, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse RBP4. You can calculate the concentration of Mouse RBP4 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-100
98-115
93-107
Average (%)
92
107
99
1:4
Range (%)
93-106
91-106
95-109
Average (%)
101
99
103
1:8
Range (%)
88-103
96-110
94-106
Average (%)
94
104
99
1:16
Range (%)
89-99
99-113
88-102
Average (%)
94
106
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
98-114
106
EDTA plasma (n=5)
93-111
101
Cell culture media (n=5)
94-109
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.93
2.64
6.9
0.89
2.46
7.21
Standard deviation
0.09
0.28
0.72
0.08
0.2
0.45
C V (%)
9.68
10.61
10.43
8.99
8.13
6.24
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse RBP4 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse RBP4 in samples. No significant cross-reactivity or interference between Mouse RBP4 and analogues was observed.
