Mouse Retinol Binding Protein 4, Plasma (RBP4) ELISA Kit
The Mouse Retinol-binding Protein 4 (Plasma RBP4) ELISA Kit is specifically designed for the accurate and sensitive detection of RBP4 levels in mouse plasma samples. This kit offers high sensitivity and specificity, ensuring precise and reliable results for a variety of research applications.Retinol-binding Protein 4 (RBP4) is a key protein involved in regulating insulin sensitivity and glucose metabolism. It is also associated with various metabolic disorders such as diabetes, obesity, and metabolic syndrome.
Monitoring RBP4 levels can provide valuable insights into these conditions and aid in the development of targeted therapies.With easy-to-follow instructions and quick assay procedures, the Mouse Retinol-binding Protein 4 (Plasma RBP4) ELISA Kit is a valuable tool for researchers studying metabolic disorders and exploring potential treatment options. Order yours today to advance your research and uncover new discoveries in the field of metabolism and disease.
Product Name:
Mouse Retinol Binding Protein 4, Plasma (RBP4) ELISA Kit
Product Code:
MODL01114
Size:
96 Assays
Target:
RBP4
Synonyms:
PRBP, RBP, Plasma retinol-binding protein
Detection Method:
Sandwich
Range:
3.12-200ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
