The Mouse RETN (Resistin) ELISA Kit is expertly designed to facilitate the precise and quantitative detection of Resistin levels across a diverse range of biological samples.
Resistin, a crucial adipokine intricately linked to metabolic regulation and inflammation, plays a vital role in modulating insulin sensitivity, glucose metabolism, and inflammatory processes within the body. Accurate measurement of Resistin levels is fundamental for unraveling its impact on metabolic homeostasis, insulin resistance, and inflammatory responses. As an essential mediator in various metabolic and inflammatory pathways, understanding the role of Resistin in disease mechanisms and its potential as a therapeutic target is of paramount importance. The Mouse RETN ELISA Kit by Assay Genie empowers researchers with exceptional sensitivity and specificity, ensuring the delivery of reliable and reproducible results. Manufactured under stringent quality control standards, this ELISA kit offers robust performance and user-friendly protocols, making it an excellent choice for researchers investigating the intricate interplay between Resistin, metabolic health, and inflammatory conditions. Advance your research with the Mouse RETN ELISA Kit and uncover novel insights into the roles of Resistin in metabolic disorders and inflammatory diseases.
Product Name:
Mouse RETN (Resistin) ELISA Kit
SKU:
AEES00248
Target:
Mouse RETN (Resistin)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
9.38 pg/ml
Detection range:
15.63-1000 pg/ml
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse RETN. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse RETN and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse RETN, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse RETN. You can calculate the concentration of Mouse RETN in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-97
96-109
94-109
Average (%)
91
102
99
1:4
Range (%)
80-91
92-105
90-103
Average (%)
86
98
96
1:8
Range (%)
81-93
91-103
94-110
Average (%)
87
97
100
1:16
Range (%)
85-100
95-108
95-107
Average (%)
91
102
101
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-108
99
EDTA plasma (n=5)
91-103
97
Cell culture media (n=5)
92-105
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
45.73
101.25
388.48
43.23
104.27
360.81
Standard deviation
2.83
4.36
14.96
2.32
5.89
16.92
C V (%)
6.18
4.31
3.85
5.37
5.65
4.69
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse RETN concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse RETN in samples. No significant cross-reactivity or interference between Mouse RETN and analogues was observed.
