The Mouse TLR4 (Toll-like Receptor 4) ELISA Kit is specifically designed for the accurate measurement of TLR4 levels in mouse serum, plasma, and tissue homogenates. This kit offers high sensitivity and specificity, ensuring reliable and consistent results for a variety of research applications.TLR4 is a key receptor in the innate immune system, playing a crucial role in detecting and responding to bacterial lipopolysaccharides. Dysregulation of TLR4 signaling has been implicated in various inflammatory diseases, making it a valuable biomarker for studying immune responses and developing targeted therapies. By utilizing the Mouse TLR4 ELISA Kit, researchers can accurately quantify TLR4 levels in biological samples, allowing for in-depth analysis of immune system function and potential therapeutic interventions. Trust in the precision and reliability of this kit to advance your research in immunology and inflammation-related studies.
Product Name:
Mouse TLR4 (Toll-Like Receptor 4) ELISA Kit
SKU:
MOES01697
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.1 ng/mL
Detection range:
0.16-10 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
