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Mouse TNF-alpha PharmaGenie ELISA Kit (For Lysates) (SBRS1542)

SKU:
SBRS1542
Product type:
ELISA Kit
Reactivity:
Mouse
Application:
ELISA
ELISA Type:
Sandwich
$792
Frequently bought together:

Description

Mouse TNF-alpha PharmaGenie ELISA Kit (For Lysates) - Kit Data

Product SKU:SBRS1542
Size:96T
Application:Cross Reactivity: This ELISA kit shows no cross-reactivity with the following cytokines tested: Mouse CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin, Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CFS, IFN-gamma, IGFBP-3, IGFBP-5, IGFBP-6, IL-1alpha, IL-1beta, IL-2, IL-3, IL-3R beta, IL-4, IL-5, IL-9, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-17, KC, Leptin R, LEPTIN (OB), LIX, L-Selectin, Lymphotactin, MCP-1, MCP-5, M-CSF, MIG, MIP-1alpha, MIP-1gamma, MIP-2, MIP-3 beta, MIP-3alpha, PF-4, P-Selectin, RANTES, SCF, SDF-1alpha, TARC, TCA-3, TECK, TIMP-1, TNF RI, TNF RII, TPO, VCAM-1, VEGF.
Uniprot:P06804
Gene ID:21926
Gene Names:TNF / TNFSF2
Synonyms:Tumor necrosis factor (Cachectin) (TNF-alpha) (Tumor necrosis factor ligand superfamily member 2) (TNF-a) [Cleaved into: Tumor necrosis factor, membrane form (N-terminal fragment) (NTF) Intracellular domain 1 (ICD1) Intracellular domain 2 (ICD2) C-domain 1 C-domain 2 Tumor necrosis factor, soluble form]
Target Species:Mouse
Compatible Sample Types:Cell Lysates, Tissue Lysates
Design Principle:Sandwich-based
Method of Detection:Colorimetric
Quantitative/Semi-Quantitative:Quantitative
Range:94 pg/ml - 6000 pg/ml
Sensitivity:94 pg/ml
Storage/Stability:The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Mouse TNF-alpha PharmaGenie ELISA Kit (For Lysates) (SBES1542) - Product Images

Product ImageStandard Curves for Mouse TNF-alpha PharmaGenie ELISA Kit (For Lysates) (SBES1542)

Kit Components:

1.Pre-Coated 96-well Strip Microplate
2.2X Cell Lysis Buffer
3.Wash Buffer
4.Stop Solution
5.Assay Diluent(s)
6.Lyophilized Standard
7.Biotinylated Detection Antibody
8.Streptavidin-Conjugated HRP
9.TMB One-Step Substrate

Other materials and equipment required:

The Assay Genie Mouse TNF-alpha ELISA (For Lysates) Kit will require other equipment and materials to carry out the assay. Please see list below for further details.

  • Distilled or deionized water
  • Precision pipettes to deliver 2 ul to 1 ul volumes
  • Adjustable 1-25 ul pipettes for reagent preparation
  • 100 ul and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis

Kit Protocol:

1.Prepare all reagents, samples and standards as instructed in the manual.
2.Add 100 ul of standard or sample to each well.
3.Incubate 2.5 h at RT or O/N at 4°C.
4.Add 100 ul of prepared biotin antibody to each well.
5.Incubate 1 h at RT.
6.Add 100 ul of prepared Streptavidin solution to each well.
7.Incubate 45 min at RT.
8.Add 100 ul of TMB One-Step Substrate Reagent to each well.
9.Incubate 30 min at RT.
10.Add 50 ul of Stop Solution to each well.
11.Read at 450 nm immediately.