The Mouse TNFRSF1A (Tumor Necrosis Factor Receptor Superfamily, Member 1A) ELISA Kit is a specialized assay crafted for the precise and quantitative detection of TNFRSF1A in various biological samples. TNFRSF1A is a pivotal member of the tumor necrosis factor receptor superfamily, playing a crucial role in modulating cellular responses to inflammatory cytokines, particularly tumor necrosis factor-alpha (TNF-α). As a central mediator of TNF-α signaling, TNFRSF1A is involved in regulating inflammation, immune responses, cell survival, and apoptosis. Accurate quantification of TNFRSF1A levels is essential for understanding its intricate functions in cellular processes, immune regulation, and disease pathogenesis. By measuring TNFRSF1A expression levels, researchers can gain valuable insights into the mechanisms underlying inflammatory conditions, autoimmune disorders, and infectious diseases where TNF-α signaling pathways are implicated. Driven by a commitment to quality and precision, the Mouse TNFRSF1A ELISA Kit from Assay Genie delivers exceptional sensitivity and specificity, ensuring reproducible and reliable results. Manufactured under stringent quality control measures, this kit offers robust performance and user-friendly protocols, making it an ideal choice for research applications aimed at unraveling the intricate roles of TNFRSF1A in biological systems.
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
