The Mouse TSH (Thyroid Stimulating Hormone) CLIA Kit is a highly sensitive and specific assay designed for the accurate measurement of TSH levels in mouse serum and plasma samples. This kit provides reliable and reproducible results, making it ideal for research applications involving thyroid function and hormone regulation.Thyroid Stimulating Hormone (TSH) is a key hormone produced by the pituitary gland that plays a crucial role in the regulation of thyroid function. Abnormal TSH levels can indicate thyroid dysfunction, making it a valuable biomarker for the study of thyroid-related conditions such as hyperthyroidism and hypothyroidism.
The Mouse TSH CLIA Kit offers researchers a powerful tool for studying the role of TSH in various physiological and pathological processes, as well as for investigating potential therapeutic interventions targeting thyroid disorders. With its high sensitivity and accuracy, this kit is an essential resource for advancing our understanding of thyroid hormone regulation in mouse models.
Product Name:
Mouse TSH (Thyroid Stimulating Hormone) CLIA Kit
SKU:
MOES00562
Target:
Mouse TSH (Thyroid Stimulating Hormone)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse TSH. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse TSH and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse TSH, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse TSH. You can calculate the concentration of Mouse TSH in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
98-113
95-107
88-99
Average (%)
104
102
94
1:4
Range (%)
103-115
96-108
101-117
Average (%)
109
102
107
1:8
Range (%)
96-111
103-115
91-105
Average (%)
103
109
97
1:16
Range (%)
99-114
93-108
93-108
Average (%)
105
99
100
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
101-112
106
EDTA plasma (n=5)
87-101
94
Cell culture media (n=5)
97-110
103
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.06
2.51
9.52
1.08
2.5
9.91
Standard deviation
0.1
0.19
0.91
0.12
0.21
0.85
C V (%)
9.43
7.57
9.56
11.11
8.4
8.58
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse TSH concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse TSH in samples. No significant cross-reactivity or interference between Mouse TSH and analogues was observed.
