Mouse VEGF-A (Vascular Endothelial Cell Growth Factor A) ELISA Kit
The Assay Genie Mouse VEGF-A (Vascular Endothelial Cell Growth Factor A) ELISA Kit is a specialized assay crafted to quantitatively detect and measure VEGF-A levels in various biological samples obtained from mouse models. VEGF-A plays a key role in promoting angiogenesis and vasculogenesis, contributing significantly to the development of new blood vessels and endothelial growth in mouse tissues and organs. Our Mouse VEGF-A ELISA Kit offers exceptional sensitivity and specificity, ensuring reliable and reproducible results essential for studying vascular development, angiogenesis, and related processes in mouse models.
Manufactured under rigorous quality control standards, this kit promises robust performance and ease of use, making it an excellent choice for research focused on investigating VEGF-A's role in biological pathways and therapeutic strategies. Trust the Assay Genie Mouse VEGF-A ELISA Kit for accurate quantification of Vascular Endothelial Growth Factor A in your research endeavors.
Product Name:
Mouse VEGF-A (Vascular Endothelial Cell Growth Factor A) ELISA Kit
SKU:
AEES00191
Target:
Mouse VEGF-A (Vascular Endothelial Cell Growth Factor A)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse VEGF-A. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse VEGF-A and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse VEGF-A, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse VEGF-A. You can calculate the concentration of Mouse VEGF-A in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-93
82-98
92-98
Average (%)
90
90
95
1:4
Range (%)
92-101
87-96
92-101
Average (%)
98
90
97
1:8
Range (%)
90-99
91-103
88-99
Average (%)
94
96
92
1:16
Range (%)
91-102
89-98
91-105
Average (%)
96
93
97
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
97-108
103
EDTA plasma (n=5)
98-108
101
Cell culture media (n=5)
99-109
104
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
94
212.07
852.78
83.63
230.4
893.36
Standard deviation
4.75
10.5
46.05
4.25
11.45
47.44
C V (%)
5.05
4.95
5.4
5.08
4.97
5.31
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse VEGF-A concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse VEGF-A in samples. No significant cross-reactivity or interference between Mouse VEGF-A and analogues was observed.
