Mouse VEGF-C (Vascular Endothelial cell Growth Factor C) ELISA Kit
The Mouse VEGF-C (Vascular Endothelial Cell Growth Factor C) ELISA Kit is a highly sensitive and specific assay designed for the accurate detection of VEGF-C levels in mouse serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it ideal for various research applications.VEGF-C is a key factor in angiogenesis, playing a crucial role in the formation of new blood vessels and regulating cell growth and survival.
Dysregulation of VEGF-C has been implicated in various diseases such as cancer, lymphedema, and inflammatory disorders, making it a valuable biomarker for studying these conditions and developing potential therapeutic interventions.With its advanced technology and user-friendly protocol, the Mouse VEGF-C ELISA Kit from Assay Genie is a valuable tool for researchers looking to study the role of VEGF-C in disease pathogenesis and progression.
Product Name:
Mouse VEGF-C (Vascular Endothelial cell Growth Factor C) ELISA Kit
SKU:
MOES01606
Target:
Mouse VEGF-C (Vascular Endothelial cell Growth Factor C)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse VEGF-C. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse VEGF-C and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse VEGF-C, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse VEGF-C. You can calculate the concentration of Mouse VEGF-C in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
97-108
87-100
98-115
Average (%)
103
92
105
1:4
Range (%)
95-110
87-97
93-106
Average (%)
102
92
98
1:8
Range (%)
93-105
82-94
91-106
Average (%)
100
87
98
1:16
Range (%)
99-113
84-97
97-111
Average (%)
105
91
103
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-106
99
EDTA plasma (n=5)
91-105
99
Cell culture media (n=5)
86-98
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
99
219.8
759.2
105.6
204.4
747.8
Standard deviation
5.5
11.9
40.2
5.7
8.8
35.9
C V (%)
5.56
5.41
5.3
5.4
4.31
4.8
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse VEGF-C concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse VEGF-C in samples. No significant cross-reactivity or interference between Mouse VEGF-C and analogues was observed.
