The Mouse VEGFR3 (FLT4) CLIA Kit is specifically designed for the quantitative measurement of vascular endothelial growth factor receptor 3 (VEGFR3) levels in mouse serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results for a variety of research applications.VEGFR3 is a vital receptor involved in mediating vascular endothelial cell growth factor signaling, playing a crucial role in angiogenesis and lymphangiogenesis processes.
Dysregulation of VEGFR3 has been implicated in various diseases such as cancer, lymphedema, and inflammatory disorders, highlighting its importance as a biomarker for studying these conditions and developing potential therapeutic interventions.Overall, the Mouse VEGFR3 (FLT4) CLIA Kit provides a reliable tool for researchers to investigate VEGFR3 levels and its role in angiogenesis-related pathways, offering valuable insights into disease mechanisms and potential therapeutic targets.
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse VEGFR3/Flt4. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse VEGFR3/Flt4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse VEGFR3/Flt4, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse VEGFR3/Flt4. You can calculate the concentration of Mouse VEGFR3/Flt4 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-112
90-104
98-112
Average (%)
102
97
106
1:4
Range (%)
93-105
101-118
102-114
Average (%)
99
109
108
1:8
Range (%)
88-100
98-115
95-107
Average (%)
95
105
102
1:16
Range (%)
85-96
98-114
93-109
Average (%)
90
104
100
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
84-95
90
EDTA plasma (n=5)
94-108
102
Cell culture media (n=5)
94-108
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
47.54
153.41
358.82
43.42
151.94
390.32
Standard deviation
4.3
14.41
32.33
4.33
17.17
27.21
C V (%)
9.05
9.39
9.01
9.97
11.3
6.97
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse VEGFR3/Flt4 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse VEGFR3/Flt4 in samples. No significant cross-reactivity or interference between Mouse VEGFR3/Flt4 and analogues was observed.
