The Mouse IL-12 ELISA Kit is a specialized assay designed for the precise quantification of Interleukin-12 (IL-12) levels in a variety of biological samples. IL-12, a crucial cytokine with potent immunoregulatory functions, plays a pivotal role in modulating the cellular activities that drive immune responses. Accurate measurement of IL-12 is essential for understanding its function in enhancing cell-mediated immunity, regulating T cell responses, and influencing various inflammatory processes. The detection and quantification of IL-12 can provide valuable insights into its role in immune system modulation and its implications in various inflammatory diseases and infections. Employing high sensitivity and specificity, the Mouse IL-12 ELISA Kit from Assay Genie ensures precise and reproducible results. Manufactured under strict quality control measures, this ELISA kit guarantees robust performance and ease of use, making it an excellent choice for research applications aimed at elucidating the intricate functions of IL-12 in immunology and inflammatory responses.
Product Name:
MouseIL-12 ELISA Kit
SKU:
AEES00251
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.17 pg/mL
Detection range:
0.31-20 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
