The MPI Antibody (CAB7319) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, has been validated for use in Western blot applications and is highly reactive with human samples.The MPI protein plays a crucial role in maintaining proper levels of vitamin B6, which is essential for various biological processes such as amino acid metabolism, neurotransmitter synthesis, and immune function. Dysregulation of MPI and vitamin B6 metabolism has been implicated in a range of diseases, including certain cancers, neurological disorders, and cardiovascular diseases.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
MPI Antibody
SKU:
CAB7319
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
PMI, PMI1, CDG1B, MPI
Positive Sample:
Jurkat, HepG2, HeLa, SW620, Mouse heart
Cellular Localization:
Cytoplasm.
Calculated MW:
47kDa
Observed MW:
47kDa
Phosphomannose isomerase catalyzes the interconversion of fructose-6-phosphate and mannose-6-phosphate and plays a critical role in maintaining the supply of D-mannose derivatives, which are required for most glycosylation reactions. Mutations in the MPI gene were found in patients with carbohydrate-deficient glycoprotein syndrome, type Ib. Alternative splicing results in multiple transcript variants.
Purification Method
Affinity purification
Gene ID
4351
RRID
AB_2767859
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using MPI Rabbit pAb (CAB7319) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
