Description
Monkeypox Virus (MPXV) A30L Protein IgG ELISA Kit
The Monkeypox Virus A30L Protein Human IgG ELISA Kit is a reliable and sensitive assay designed for the accurate detection of Monkeypox Virus A30L Protein-specific IgG antibodies in human serum, plasma, and cell culture supernatants. This kit offers high specificity and precision, ensuring consistent and reproducible results for a variety of research purposes.The Monkeypox Virus A30L Protein is a vital component of the Monkeypox virus, which is a close relative of the smallpox virus and causes a similar but less severe disease in humans.
Detection of specific IgG antibodies against this protein can be crucial for monitoring Monkeypox virus infections and immunity, as well as for vaccine development and epidemiological studies.This ELISA kit provides researchers with a valuable tool for studying Monkeypox virus infections, immune responses, and potential therapeutic interventions. Its user-friendly format and accurate results make it a valuable asset for laboratories and research institutions focusing on infectious diseases and virology.
| Product Name: | Monkeypox Virus (MPXV) A30L Protein IgG ELISA Kit |
| Product Code: | MKRS0006 |
| Species Detected: | Human |
| Gene Symbols: | IGG |
| Compatible Sample Types: | Plasma, Serum |
| Design Principle: | Indirect |
| Number of Targets Detected: | 1 |
| Method of Detection: | Colorimetric |
| Quantitative/Semi-Quantitative: | Quantitative |
| Solid Support: | 96-well Microplate |
| Size: | 96T |
- Pre-coated 96-well microplate
- Wash Buffer
- Positive Control
- Biotinylated Detection Antibody
- Biotinylated Anti-Positive Control
- HRP-Streptavidin concentrate
- TMB One-Step Substrate
- Stop Solution
- Assay Diluent
- 5X Sample Diluent
Other Materials Required
- Microplate reader capable of measuring absorbance at 450 nm
- Precision pipettes to deliver 2 µl to 1 ml volumes
- Adjustable 1-25 ml pipettes for reagent preparation
- 100 ml and 1 liter graduated cylinders
- Absorbent paper
- Distilled or deionized water
- Prepare all reagents, samples and standards as instructed.
- Add 100 µl positive control or sample to each well. Incubate 1 hour at room temperature.
- Add 100 µl prepared Biotinylated detection antibody and Biotinylated Anti-Postive Control into respective wells. Incubate 1 hour at room temperature.
- Add 100 µl prepared HRP-Streptavidin solution to each well. Incubate 30 minutes at room temperature.
- Add 100 µl TMB One-Step Substrate Reagent to each well. Incubate 30 minutes at room temperature.
- Add 50 µl Stop Solution to each well. Read at 450 nm immediately.
