Description
Monkeypox Virus (MPXV) A33R Protein IgM ELISA Kit
The Monkeypox Virus A33R Protein Human IgM ELISA Kit is a cutting-edge tool for the detection of IgM antibodies specific to the A33R protein of the Monkeypox virus in human serum, plasma, and other biological samples. This kit offers unparalleled sensitivity and specificity, ensuring accurate and reproducible results for researchers and healthcare professionals.The A33R protein plays a crucial role in the pathogenesis of Monkeypox virus infection by enabling viral entry into host cells and evading the immune response. Detection of IgM antibodies against A33R can provide valuable insights into the immune response to Monkeypox virus infection, aiding in diagnosis, monitoring disease progression, and assessing vaccine effectiveness.
With its advanced technology and user-friendly design, the Monkeypox Virus A33R Protein Human IgM ELISA Kit is an indispensable tool for studying Monkeypox virus pathogenesis, developing diagnostic assays, and evaluating potential therapeutics. Whether in the laboratory or clinical setting, this kit is a valuable resource for advancing research and improving patient care in the field of virology.
| Product Name: | Monkeypox Virus (MPXV) A33R Protein IgM ELISA Kit |
| Product Code: | MKRS0010 |
| Species Detected: | Human |
| Gene Symbols: | IGM |
| Compatible Sample Types: | Plasma, Serum |
| Design Principle: | Indirect |
| Number of Targets Detected: | 1 |
| Method of Detection: | Colorimetric |
| Quantitative/Semi-Quantitative: | Quantitative |
| Solid Support: | 96-well Microplate |
| Size: | 96T |
- Pre-coated 96-well microplate
- Wash Buffer
- Positive Control
- Biotinylated Detection Antibody
- Biotinylated Anti-Positive Control
- HRP-Streptavidin concentrate
- TMB One-Step Substrate
- Stop Solution
- Assay Diluent
- 5X Sample Diluent
Other Materials Required
- Microplate reader capable of measuring absorbance at 450 nm
- Precision pipettes to deliver 2 µl to 1 ml volumes
- Adjustable 1-25 ml pipettes for reagent preparation
- 100 ml and 1 liter graduated cylinders
- Absorbent paper
- Distilled or deionized water
- Prepare all reagents, samples and standards as instructed.
- Add 100 µl positive control or sample to each well. Incubate 1 hour at room temperature.
- Add 100 µl prepared Biotinylated detection antibody and Biotinylated Anti-Postive Control into respective wells. Incubate 1 hour at room temperature.
- Add 100 µl prepared HRP-Streptavidin solution to each well. Incubate 30 minutes at room temperature.
- Add 100 µl TMB One-Step Substrate Reagent to each well. Incubate 30 minutes at room temperature.
- Add 50 µl Stop Solution to each well. Read at 450 nm immediately.
