Description
Monkeypox Virus (MPXV) B16R Protein IgM ELISA Kit
The Monkeypox Virus B16R Protein Human IgM ELISA Kit is a reliable and accurate tool for the detection of Monkeypox Virus B16R protein-specific IgM antibodies in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring robust and reproducible results for various research applications.The Monkeypox Virus B16R protein is a critical antigen involved in the immune response to Monkeypox Virus infection. By detecting IgM antibodies specific to this protein, researchers can gain valuable insights into the immune response to Monkeypox Virus and monitor the progression of infection.
This ELISA kit is essential for studying Monkeypox Virus infection, understanding the immune response, and developing potential therapeutics and vaccines. With its high-quality components and easy-to-follow protocol, the Monkeypox Virus B16R Protein Human IgM ELISA Kit is a valuable asset for researchers in the field of virology and infectious diseases.
| Product Name: | Monkeypox Virus (MPXV) B16R Protein IgM ELISA Kit |
| Product Code: | MKRS0016 |
| Species Detected: | Human |
| Gene Symbols: | IGM |
| Compatible Sample Types: | Plasma, Serum |
| Design Principle: | Indirect |
| Number of Targets Detected: | 1 |
| Method of Detection: | Colorimetric |
| Quantitative/Semi-Quantitative: | Quantitative |
| Solid Support: | 96-well Microplate |
| Size: | 96T |
- Pre-coated 96-well microplate
- Wash Buffer
- Positive Control
- Biotinylated Detection Antibody
- Biotinylated Anti-Positive Control
- HRP-Streptavidin concentrate
- TMB One-Step Substrate
- Stop Solution
- Assay Diluent
- 5X Sample Diluent
Other Materials Required
- Microplate reader capable of measuring absorbance at 450 nm
- Precision pipettes to deliver 2 µl to 1 ml volumes
- Adjustable 1-25 ml pipettes for reagent preparation
- 100 ml and 1 liter graduated cylinders
- Absorbent paper
- Distilled or deionized water
- Prepare all reagents, samples and standards as instructed.
- Add 100 µl positive control or sample to each well. Incubate 1 hour at room temperature.
- Add 100 µl prepared Biotinylated detection antibody and Biotinylated Anti-Postive Control into respective wells. Incubate 1 hour at room temperature.
- Add 100 µl prepared HRP-Streptavidin solution to each well. Incubate 30 minutes at room temperature.
- Add 100 µl TMB One-Step Substrate Reagent to each well. Incubate 30 minutes at room temperature.
- Add 50 µl Stop Solution to each well. Read at 450 nm immediately.
