The MRP4/ABCC4 Polyclonal Antibody (CAB21322) is a high-quality antibody developed for reliable detection and analysis of target proteins. MRP4, a member of the ATP-binding cassette (ABC) family of transporters, is involved in the transport of a wide range of molecules across cell membranes. This antibody, generated in rabbits, exhibits high specificity and sensitivity for detecting MRP4 in human samples, making it ideal for use in Western blot applications.MRP4 has been implicated in drug resistance in cancer cells, as well as in the regulation of intracellular levels of signaling molecules and toxins.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
MRP4/ABCC4 Polyclonal Antibody
SKU:
CAB21322
Size:
20μL, 100μL
Reactivity:
Human
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
MRP4, MOATB, MOAT-B, MRP4/ABCC4
Positive Sample:
HeLa
Cellular Localization:
Membrane, Multi-Pass Membrane Protein.
Calculated MW:
150kDa
Observed MW:
140-200kDa
The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MRP subfamily which is involved in multi-drug resistance. This family member plays a role in cellular detoxification as a pump for its substrate, organic anions. It may also function in prostaglandin-mediated cAMP signaling in ciliogenesis. Alternative splicing of this gene results in multiple transcript variants.
Purification Method
Affinity purification
Gene ID
10257
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from HeLa cells, using MRP4/ABCC4 Rabbit pAb (CAB21322) at 1:1000 dilution. membrane protein extract isolated from Hela Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
