The MRPL1 Antibody (CAB4947) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. It specifically binds to the MRPL1 protein, enabling accurate detection and analysis in a variety of cell types.MRPL1 is a key player in mitochondrial function, participating in the assembly of the mitochondrial ribosome and translation of mitochondrial-encoded proteins. Dysregulation of MRPL1 has been linked to mitochondrial dysfunction, which is implicated in various diseases including cancer, neurodegenerative disorders, and metabolic conditions.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Rat samples.
Product Name:
MRPL1 Antibody
SKU:
CAB4947
Size:
20μL, 100μL
Reactivity:
Human, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
L1MT, BM022, MRP-L1, MRPL1
Positive Sample:
A-431, MCF7, HeLa, Jurkat
Cellular Localization:
Mitochondrion.
Calculated MW:
37kDa
Observed MW:
37kDa
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein that belongs to the L1 ribosomal protein family.
Purification Method
Affinity purification
Gene ID
65008
RRID
AB_2765947
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using MRPL1 Rabbit pAb (CAB4947) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
