The Mouse IL-12 p40 ELISA Kit - High Sensitivity is an innovative assay designed for the precise and sensitive quantification of Interleukin-12 p40 (IL-12 p40) levels in a variety of biological samples obtained from mouse models. IL-12 p40 is a crucial cytokine that plays a significant role in modulating immune responses and inflammation, contributing to cell activation, differentiation, and immune system regulation. Its involvement in pro-inflammatory responses underscores its importance in various immune-mediated diseases, autoimmune conditions, and infection-related pathways. This state-of-the-art ELISA kit offers exceptional sensitivity and specificity, allowing researchers to accurately measure IL-12 p40 concentrations. Understanding the levels of IL-12 p40 is essential for elucidating its role in immune system regulation, inflammatory processes, and disease pathogenesis, providing valuable insights for targeted therapeutic interventions. Manufactured under rigorous quality control measures, the Mouse IL-12 p40 ELISA Kit - High Sensitivity ensures robust and reproducible performance, making it an ideal choice for cutting-edge research applications. Its user-friendly design and reliable performance empower researchers to explore the intricate roles of IL-12 p40 in immune responses and inflammatory conditions with precision and confidence. Uncover the intricacies of IL-12 p40 biology using the Mouse IL-12 p40 ELISA Kit - High Sensitivity from Assay Genie.
Product Name:
Mouse IL-12 p40 ELISA Kit (High Sensitivity)
SKU:
AEES00628
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
