Description
Mouse IL-2 ELISA Kit (High Sensitivity)
The Mouse IL-2 ELISA Kit - High Sensitivity is a cutting-edge assay designed for the precise and sensitive quantitation of Interleukin-2 (IL-2) levels in diverse mouse biological samples. IL-2, a vital cytokine, plays a fundamental role in modulating immune responses, particularly in the regulation of T cell proliferation, survival, and differentiation. Its intricate involvement in immune activation, tolerance, and homeostasis highlights its significance in orchestrating cellular immunity and immune-mediated processes. This high-sensitivity ELISA kit enables researchers to delve into the immunological landscape by accurately measuring IL-2 levels, offering key insights into the dynamic immune responses underlying inflammatory reactions, autoimmunity, infectious diseases, and immune-related disorders.
Understanding the nuanced functions of IL-2 is essential for deciphering the complexities of immune regulation and developing targeted therapeutic interventions to modulate immune responses effectively. With exceptional sensitivity and specificity, the Mouse IL-2 ELISA Kit - High Sensitivity from Assay Genie ensures dependable and reproducible results, empowering researchers with a robust tool to explore the critical role of IL-2 in immune homeostasis and disease pathogenesis. Manufactured under stringent quality control standards, this kit delivers optimal performance and user-friendly protocols, making it an invaluable asset for in-depth immunological research and drug development endeavors.
| Product Name: | Mouse IL-2 ELISA Kit (High Sensitivity) |
| Product Code: | AEES00623 |
| Assay Type: | Sandwich |
| Format: | 96 Assays |
| Assay Time: | 3.5h |
| Reactivity: | Mouse |
| Detection Range: | 7.81-500 pg/mL |
| Sensitivity: | 4.69 pg/mL |
| Sample Type & Sample Volume: | Serum, plasma and other biological fluids, 25?L |
| Specificity: | This kit recognizes Mouse IL-2 in samples. No significant cross-reactivity or interference between Mouse IL-2 and analogues was observed. |
| Reproducibility: | Both intra-CV and inter-CV are < 10%. |
| Application: | This ELISA kit applies to the in vitro quantitative determination of Mouse IL-2 concentrations in serum, plasma and other biological fluids. |
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse IL-2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse IL-2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse IL-2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse IL-2. You can calculate the concentration of Mouse IL-2 in the samples by comparing the OD of the samples to the standard curve.
| Kit Components: | An unopened kit can be stored at 2-8? for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
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