Description
Mouse MCP-1/CCL-2 ELISA Kit (High Sensitivity)
**** The Mouse MCP-1 (Monocyte Chemotactic Protein 1) ELISA Kit - High Sensitivity is a state-of-the-art assay engineered for the precise quantitative measurement of MCP-1 levels in various biological samples from mouse models. As a key cytokine involved in immune responses and inflammatory processes, MCP-1 plays a crucial role in monocyte chemotaxis, immune cell recruitment, and inflammatory cascade modulation, making it a significant target for research elucidating immune-related disorders, inflammatory conditions, and disease progression in mouse models.
This high-sensitivity ELISA kit is meticulously designed to provide researchers with exceptional accuracy in assessing MCP-1 expression levels, enabling detailed investigations into its role in immune cell migration, cytokine signaling, and inflammatory pathways with unparalleled sensitivity. The enhanced sensitivity of this kit empowers researchers to explore the intricate mechanisms of MCP-1 in immune responses and inflammatory processes in mouse models with utmost precision and reliability. The Mouse MCP-1 ELISA Kit - High Sensitivity by Assay Genie ensures exceptional sensitivity and specificity, guaranteeing reliable and reproducible results. Manufactured under rigorous quality control standards, this kit delivers robust performance and ease of use, making it the preferred choice for researchers seeking to unravel the complexities of Monocyte Chemotactic Protein 1 in immune responses and inflammatory cascades in mouse models.
| Product Name: | Mouse MCP-1/CCL-2 ELISA Kit (High Sensitivity) |
| Product Code: | AEES00599 |
| Assay Type: | Sandwich |
| Format: | 96T |
| Assay Time: | 3.5h |
| Reactivity: | Mouse |
| Detection Range: | 15.63-1000 pg/mL |
| Sensitivity: | 9.38 pg/mL |
| Sample Type & Sample Volume: | Serum, plasma and other biological fluids, 25?L |
| Specificity: | This kit recognizes Mouse MCP-1 in samples. No significant cross-reactivity or interference between Mouse MCP-1 and analogues was observed. |
| Reproducibility: | Both intra-CV and inter-CV are < 10%. |
| Application: | This ELISA kit applies to the in vitro quantitative determination of Mouse MCP-1 concentrations in serum, plasma and other biological fluids. |
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse MCP-1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse MCP-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse MCP-1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse MCP-1. You can calculate the concentration of Mouse MCP-1 in the samples by comparing the OD of the samples to the standard curve.
| Kit Components: | An unopened kit can be stored at 2-8? for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
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