Description
Mouse Osteoprotegerin (TNFRSF11B) ELISA Kit (High Sensitivity)
The Mouse OPG (Osteoprotegerin) ELISA Kit - High Sensitivity is a specialized assay designed for the quantitative measurement of Osteoprotegerin (OPG) levels in various mouse biological samples with exceptional sensitivity. OPG, a key regulator of bone metabolism and a member of the tumor necrosis factor receptor superfamily, is critically involved in bone remodeling, osteoclast regulation, and vascular calcification processes. As a decoy receptor for RANKL (Receptor Activator of Nuclear Factor Kappa-B Ligand), OPG plays a significant role in inhibiting osteoclast differentiation and activity, thus influencing bone homeostasis and vascular health. By offering high sensitivity and precision, the Mouse OPG ELISA Kit enables researchers to elucidate the intricate mechanisms underlying bone metabolism and vascular health.
Accurate quantification of OPG levels is essential for gaining insights into bone-related conditions, such as osteoporosis, osteopetrosis, and skeletal disorders, as well as vascular diseases involving calcification. With a focus on reliability and reproducibility, this ELISA kit ensures trustworthy and consistent results, empowering researchers to investigate the biological functions and therapeutic implications of OPG with confidence. Manufactured under stringent quality standards and designed for user-friendly operation, this kit from Assay Genie provides robust performance, making it an indispensable tool for bone research, vascular health studies, and drug development endeavors.
| Product Name: | Mouse Osteoprotegerin (TNFRSF11B) ELISA Kit (High Sensitivity) |
| Product Code: | AEES00625 |
| Assay Type: | Sandwich |
| Format: | 96 Assays |
| Assay Time: | 3.5h |
| Reactivity: | Mouse |
| Detection Range: | 125.00-8000 pg/mL |
| Sensitivity: | 62.87 pg/mL |
| Sample Type & Sample Volume: | Serum, plasma and other biological fluids, 25?L |
| Specificity: | This kit recognizes Mouse OPG in samples. No significant cross-reactivity or interference between Mouse OPG and analogues was observed. |
| Reproducibility: | Both intra-CV and inter-CV are < 10%. |
| Application: | This ELISA kit applies to the in vitro quantitative determination of Mouse OPG concentrations in serum, plasma and other biological fluids. |
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse OPG. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse OPG and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse OPG, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse OPG. You can calculate the concentration of Mouse OPG in the samples by comparing the OD of the samples to the standard curve.
| Kit Components: | An unopened kit can be stored at 2-8? for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
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