The Mouse SELE (E-selectin) ELISA Kit - High Sensitivity is a cutting-edge assay designed for the precise quantitative detection of E-selectin levels in various biological samples sourced from mouse models. E-selectin, a cell adhesion molecule, plays a crucial role in mediating leukocyte-endothelial cell interactions, making it a key molecule in elucidating inflammatory responses, vascular dynamics, and immune system regulation in mouse studies. This high-sensitivity ELISA kit provides researchers with a powerful tool to explore E-selectin dynamics with exceptional accuracy and sensitivity. By offering a detailed insight into the expression levels of E-selectin, researchers can unravel its involvement in inflammatory processes, adherence mechanisms, and vascular homeostasis with unprecedented precision. The heightened sensitivity of this kit allows for in-depth investigations into the intricate functions and regulatory pathways governed by E-selectin in mouse models. The Mouse SELE ELISA Kit - High Sensitivity by Assay Genie ensures outstanding sensitivity and specificity, guaranteeing reliable and reproducible results. Manufactured under strict quality control standards, this kit delivers robust performance and straightforward usability, making it an ideal choice for researchers seeking to uncover the roles and functions of E-selectin in mouse models.
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
