Description
Mouse TNF-alpha ELISA Kit (High Sensitivity)
**** The Mouse TNF-? (Tumor Necrosis Factor Alpha) ELISA Kit - High Sensitivity is a cutting-edge assay designed for the precise and quantitative measurement of Tumor Necrosis Factor Alpha (TNF-?) levels in various mouse biological samples.
TNF-? is a critical pro-inflammatory cytokine that plays a pivotal role in various cellular processes such as inflammation, cell proliferation, immune response modulation, and cell death. This high-sensitivity ELISA kit offers unparalleled accuracy and reliability in TNF-? detection, enabling researchers to delve into the intricate roles of TNF-? in disease mechanisms, autoimmune disorders, cancer, and infections. With exceptional sensitivity and specificity, this kit provides reproducible results, empowering scientists to explore the complexities of TNF-? biology and develop targeted therapeutic strategies. Manufactured under stringent quality control standards, the Mouse TNF-? ELISA Kit - High Sensitivity delivers robust performance and user-friendly protocols, making it an indispensable tool for cutting-edge research in immunology, inflammation, and cytokine biology utilizing mouse models.
| Product Name: | Mouse TNF-alpha ELISA Kit (High Sensitivity) |
| Product Code: | AEES00589 |
| Assay Type: | Sandwich |
| Format: | 96T |
| Assay Time: | 3.5h |
| Reactivity: | Mouse |
| Detection Range: | 7.81-500 pg/mL |
| Sensitivity: | 4.69 pg/mL |
| Sample Type & Sample Volume: | Serum, plasma and other biological fluids, 25?L |
| Specificity: | This kit recognizes Mouse TNF-? in samples. No significant cross-reactivity or interference between Mouse TNF-? and analogues was observed. |
| Reproducibility: | Both intra-CV and inter-CV are < 10%. |
| Application: | This ELISA kit applies to the in vitro quantitative determination of Mouse TNF-? concentrations in serum, plasma and other biological fluids. |
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse TNF-?. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse TNF-? and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse TNF-?, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse TNF-?. You can calculate the concentration of Mouse TNF-? in the samples by comparing the OD of the samples to the standard curve.
| Kit Components: | An unopened kit can be stored at 2-8? for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
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