The MT-ATP8 Antibody (CAB17890) is a high-quality antibody developed for reliable detection and analysis of target proteins. Contributes to proton-transporting ATP synthase activity, rotational mechanism. Involved in mitochondrial ATP synthesis coupled proton transport. Part of mitochondrial proton-transporting ATP synthase complex. Implicated in multiple sclerosis and urinary bladder cancer.
This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
MT-ATP8 Antibody
SKU:
CAB17890
Size:
100μL, 20μL
Reactivity:
Human, Mouse, Rat
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBIHC-PIF/ICCELISA
Recommended Dilution:
WB
1:500 - 1:2000
IHC-P
1:50 - 1:100
IF/ICC
1:50 - 1:100
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
ATPase8, MTATP8, ATP8, MT-ATP8
Positive Sample:
HeLa treated with EB, Rat liver, Hep G2
Cellular Localization:
Mitochondrial Inner Membrane, Mitochondrial Proton-Transporting Atp Synthase Complex, Mitochondrial Proton-Transporting Atp Synthase Complex, Coupling Factor F(O).
Calculated MW:
8 kDa
Observed MW:
8 kDa
Contributes to proton-transporting ATP synthase activity, rotational mechanism. Involved in mitochondrial ATP synthesis coupled proton transport. Part of mitochondrial proton-transporting ATP synthase complex. Implicated in multiple sclerosis and urinary bladder cancer.
Purification Method
Affinity purification
Gene ID
4509
RRID
AB_2861745
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using MT-ATP8 Rabbit pAb (CAB17890) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Immunohistochemistry analysis of paraffin-embedded Human liver tissue using MT-ATP8 Rabbit pAb (CAB17890) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Immunofluorescence analysis of HeLa cells using MT-ATP8 Rabbit pAb (CAB17890) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
