The Munc 13-1 Polyclonal Antibody (CAB22550) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced through immunization of rabbits, is highly specific and reacts strongly with human samples. Validated for use in Western blotting applications, it allows for the detection and analysis of Munc 13-1 in various cell types, making it an essential asset for studies in neuroscience and neurobiology.Munc 13-1 is a key player in the regulation of synaptic transmission and neurotransmitter release, essential processes for proper brain function and communication between neurons.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Munc 13-1 Polyclonal Antibody
SKU:
CAB22550
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:1000 - 1:5000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
Munc13-1, Munc 13-1
Positive Sample:
Mouse brain, Rat brain
Cellular Localization:
Neuromuscular Junction, Neuron Projection, Plasma Membrane, Presynaptic Active Zone, Presynaptic Active Zone Cytoplasmic Component, Presynaptic Membrane, Synaptic Vesicle Membrane, Terminal Bouton.
Calculated MW:
193kDa
Observed MW:
220kDa
This gene encodes a member of the UNC13 family. UNC13 proteins bind to phorbol esters and diacylglycerol and play important roles in neurotransmitter release at synapses. Single nucleotide polymorphisms in this gene may be associated with sporadic amyotrophic lateral sclerosis.
Purification Method
Affinity purification
Gene ID
23025
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates, using Munc 13-1 Rabbit pAb (CAB22550) at 1:2000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
