The MYH7/beta-MHC Monoclonal Antibody (CAB22140) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced using hybridoma technology, specifically targets the MYH7 protein and is ideal for use in various applications such as Western blot, immunofluorescence, and immunohistochemistry.The MYH7 protein is predominantly expressed in cardiac and skeletal muscle tissues, playing a crucial role in muscle contraction and overall muscle function. Mutations in the MYH7 gene have been linked to various muscle disorders, including cardiomyopathy and skeletal muscle myopathies.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
MYH7/beta-MHC Monoclonal Antibody
SKU:
CAB22140
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC55749
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Muscle myosin is a hexameric protein containing 2 heavy chain subunits, 2 alkali light chain subunits, and 2 regulatory light chain subunits. This gene encodes the beta (or slow) heavy chain subunit of cardiac myosin. It is expressed predominantly in normal human ventricle. It is also expressed in skeletal muscle tissues rich in slow-twitch type I muscle fibers. Changes in the relative abundance of this protein and the alpha (or fast) heavy subunit of cardiac myosin correlate with the contractile velocity of cardiac muscle. Its expression is also altered during thyroid hormone depletion and hemodynamic overloading. Mutations in this gene are associated with familial hypertrophic cardiomyopathy, myosin storage myopathy, dilated cardiomyopathy, and Laing distal myopathy.
Purification Method
Affinity purification
Gene ID
4625
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of various lysates using MYH7/β-MHC Rabbit mAb (CAB22140) at 1:2000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 4s.
