The MYL9 Monoclonal Antibody (CAB8738) is a high-quality antibody developed for reliable detection and analysis of target proteins. Myosin, a structural component of muscle, consists of two heavy chains and four light chains. The protein encoded by this gene is a myosin light chain that may regulate muscle contraction by modulating the ATPase activity of myosin heads. The encoded protein binds calcium and is activated by myosin light chain kinase. Two transcript variants encoding different isoforms have been found for this gene.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
MYL9 Monoclonal Antibody
SKU:
CAB8738
Size:
100μL, 20μL
Reactivity:
Human, Mouse
Clone Number:
ARC1284
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:1000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
LC20, MLC2, MRLC1, MYRL2, MLC-2C, MMIHS4, MYL9
Positive Sample:
HeLa, C2C12, Mouse lung
Cellular Localization:
Cell Cortex, Cytoplasm, Cytosol, Myofibril, Z Disc.
Calculated MW:
20kDa
Observed MW:
20kDa
Myosin, a structural component of muscle, consists of two heavy chains and four light chains. The protein encoded by this gene is a myosin light chain that may regulate muscle contraction by modulating the ATPase activity of myosin heads. The encoded protein binds calcium and is activated by myosin light chain kinase. Two transcript variants encoding different isoforms have been found for this gene.
Purification Method
Affinity purification
Gene ID
10398
RRID
AB_2863597
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using MYL9 Rabbit mAb (CAB8738) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
