The N-Myc/MYCN Monoclonal Antibody (CAB22175) is a vital tool for researchers studying the N-Myc/MYCN protein, which plays a crucial role in cell proliferation and differentiation, particularly in neuroblastoma. This monoclonal antibody, generated in mice, is highly specific to human N-Myc/MYCN samples and has been validated for use in various research applications including Western blot and immunohistochemistry.N-Myc/MYCN is a transcription factor that is frequently amplified in neuroblastoma, a type of childhood cancer that arises in immature nerve cells.
Understanding the function of N-Myc/MYCN is essential for investigating the molecular mechanisms underlying neuroblastoma progression and for identifying potential therapeutic targets. This antibody enables the detection and characterization of N-Myc/MYCN protein expression in tumor samples, paving the way for advancements in cancer research and the development of targeted therapies for neuroblastoma patients.
Product Name:
N-Myc/MYCN Monoclonal Antibody
SKU:
CAB22175
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Mouse
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human N-Myc/MYCN (NP_005369.2).
This gene is a member of the MYC family and encodes a protein with a basic helix-loop-helix (bHLH) domain. This protein is located in the nucleus and must dimerize with another bHLH protein in order to bind DNA. Amplification of this gene is associated with a variety of tumors, most notably neuroblastomas. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
4613
Clone Number:
ARC55217
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of lysates from Neuro-2a, cells using N-Myc/MYCN Rabbit mAb (CAB22175) at1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 180s.
