The N6AMT1/HEMK2 Polyclonal Antibody (CAB21784) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, is highly specific and reacts strongly with human samples, making it ideal for use in Western blot applications.The N6amt1/HemK2 protein is essential for the methylation of specific tRNAs, which is crucial for accurate protein synthesis. Dysregulation of this process has been linked to various diseases, including cancer and neurological disorders.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
N6AMT1/HEMK2 Polyclonal Antibody
SKU:
CAB21784
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
This gene encodes an N(6)-adenine-specific DNA methyltransferase. The encoded enzyme may be involved in the methylation of release factor I during translation termination. This enzyme is also involved in converting the arsenic metabolite monomethylarsonous acid to the less toxic dimethylarsonic acid. Alternative splicing of this gene results in multiple transcript variants. A related pseudogene has been identified on chromosome 11.
Purification Method
Affinity purification
Gene ID
29104
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates, using N6AMT1 Rabbit pAb (CAB21784) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer:3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: Q120s.
Immunohistochemistry analysis of paraffin-embedded Human thyroid cancer tissue using N6AMT1/HEMK2 Rabbit pAb (CAB21784) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
