The p47phox/NCF1 Antibody (CAB1148) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced from rabbits, is highly specific to human samples and has been validated for use in Western blot applications. By binding to the NCF1 protein, researchers can detect and analyze NCF1 levels in various cell types, making it a valuable tool for studies in immunology and related fields.NCF1, also known as neutrophil cytosolic factor 1, plays a crucial role in the production of reactive oxygen species in phagocytes, which are essential for microbial killing.
This antibody is validated for use in WB, IF/ICC, IP, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
p47phox/NCF1 Antibody
SKU:
CAB1148
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
The protein encoded by this gene is a 47 kDa cytosolic subunit of neutrophil NADPH oxidase. This oxidase is a multicomponent enzyme that is activated to produce superoxide anion. Mutations in this gene have been associated with chronic granulomatous disease.
Purification Method
Affinity purification
Gene ID
653361
RRID
AB_2758579
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates, using p47phox/NCF1 Rabbit pAb (CAB1148) at 1:400 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
Western blot analysis of lysates from Raji cells using p47phox/NCF1 Rabbit pAb (CAB1148) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time:10s.
