The NDUFA8 Antibody (CAB12118) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high reactivity specifically with human samples and is validated for use in Western blot applications.NDUFA8 is a key player in mitochondrial energy production and function, making it a crucial target for research on metabolic disorders, neurodegenerative diseases, and cancer. By detecting and analyzing NDUFA8 levels in various cell types, this antibody enables researchers to gain insights into the role of this protein in cellular energetics and disease pathogenesis.
This antibody is validated for use in WB, IF/ICC, IP, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
NDUFA8 Antibody
SKU:
CAB12118
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
The protein encoded by this gene belongs to the complex I 19 kDa subunit family. Mammalian complex I is composed of 45 different subunits. This protein has NADH dehydrogenase activity and oxidoreductase activity. It plays an important role in transfering electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms.
Purification Method
Affinity purification
Gene ID
4702
RRID
AB_2759008
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using NDUFA8 Rabbit pAb (CAB12118) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
Immunofluorescence analysis of H9C2 cells using NDUFA8 Rabbit pAb (CAB12118) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200 μg extracts of 293T cells, using 3 μg NDUFA8 antibody (CAB12118). Western blot was performed from the immunoprecipitate using NDUFA8 antibody (CAB12118) at a dilution of 1:1000.
