The NEK2 Monoclonal Antibody (CAB6811) is a high-quality antibody developed for reliable detection and analysis of target proteins. This gene encodes a serine/threonine-protein kinase that is involved in mitotic regulation. This protein is localized to the centrosome, and undetectable during G1 phase, but accumulates progressively throughout the S phase, reaching maximal levels in late G2 phase. Alternatively spliced transcript variants encoding different isoforms with distinct C-termini have been noted for this gene.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
NEK2 Monoclonal Antibody
SKU:
CAB6811
Size:
100μL, 20μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC1418
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:2000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
NLK1, RP67, NEK2A, HsPK21, PPP1R111, NEK2
Positive Sample:
PC-3, Mouse testis, Rat testis
Cellular Localization:
Chromosome, Cytoplasm, Nucleus, Centromere, Centromere, Centrosome, Cytoskeleton, Kinetochore, Microtubule Organizing Center, Nucleolus, Spindle Pole.
Calculated MW:
52kDa
Observed MW:
50kDa
This gene encodes a serine/threonine-protein kinase that is involved in mitotic regulation. This protein is localized to the centrosome, and undetectable during G1 phase, but accumulates progressively throughout the S phase, reaching maximal levels in late G2 phase. Alternatively spliced transcript variants encoding different isoforms with distinct C-termini have been noted for this gene.
Purification Method
Affinity purification
Gene ID
4751
RRID
AB_2863536
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using NEK2 Rabbit mAb (CAB6811) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
