The Notch1 Polyclonal Antibody (CAB18282) is a valuable tool for researchers studying the Notch1 protein, a key regulator of cellular processes such as cell differentiation, proliferation, and apoptosis. This antibody, produced in rabbits, is highly specific for Notch1 and has been validated for use in Western blot applications.Notch1 is a transmembrane receptor involved in various developmental pathways and is frequently dysregulated in cancer, making it a promising target for cancer therapies. By binding to Notch1 protein, this antibody allows for accurate detection and analysis in a variety of cell types, making it ideal for studies in developmental biology and cancer research.
Understanding the role of Notch1 in cellular processes and disease pathogenesis is essential for developing targeted therapies and advancing our knowledge of Notch signaling in health and disease. The Notch1 Polyclonal Antibody is a reliable tool for researchers investigating the complex mechanisms regulated by Notch1 and its implications in various cellular processes.
Product Name:
Notch1 Rabbit Polyclonal Antibody
SKU:
CAB18282
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1567-1729 of human Notch1 (NP_060087.3).
Cell membrane, Nucleus, Single-pass type I membrane protein.
Calculated MW:
273kDa
Observed MW:
120kDa
This gene encodes a member of the NOTCH family of proteins. Members of this Type I transmembrane protein family share structural characteristics including an extracellular domain consisting of multiple epidermal growth factor-like (EGF) repeats, and an intracellular domain consisting of multiple different domain types. Notch signaling is an evolutionarily conserved intercellular signaling pathway that regulates interactions between physically adjacent cells through binding of Notch family receptors to their cognate ligands. The encoded preproprotein is proteolytically processed in the trans-Golgi network to generate two polypeptide chains that heterodimerize to form the mature cell-surface receptor. This receptor plays a role in the development of numerous cell and tissue types. Mutations in this gene are associated with aortic valve disease, Adams-Oliver syndrome, T-cell acute lymphoblastic leukemia, chronic lymphocytic leukemia, and head and neck squamous cell carcinoma.
Purification Method:
Affinity purification
Gene ID:
4851
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of lysates from Jurkat cells, using Notch1 Rabbit pAb (CAB18282) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
