The NOX2/gp91phox Monoclonal Antibody (CAB19701) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, has been extensively validated for use in Western blot applications with human samples.The NOX2/Cybb protein, also known as GP91phox, plays a critical role in the antimicrobial activity of phagocytes by generating superoxide radicals to kill engulfed pathogens. Dysregulation of NOX2/Cybb activity is associated with various diseases, including chronic granulomatous disease (CGD), inflammatory conditions, and cancer.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
NOX2/gp91phox Monoclonal Antibody
SKU:
CAB19701
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC0181
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Mouse spleen, Mouse heart, Rat lung, Rat spleen, HepG2, Raji, HL-60, Mouse lung
Cellular Localization:
Cell Membrane, Multi-Pass Membrane Protein.
Calculated MW:
65kDa
Observed MW:
65kDa
Cytochrome b (-245) is composed of cytochrome b alpha (CYBA) and beta (CYBB) chain. It has been proposed as a primary component of the microbicidal oxidase system of phagocytes. CYBB deficiency is one of five described biochemical defects associated with chronic granulomatous disease (CGD). In this disorder, there is decreased activity of phagocyte NADPH oxidase; neutrophils are able to phagocytize bacteria but cannot kill them in the phagocytic vacuoles. The cause of the killing defect is an inability to increase the cell's respiration and consequent failure to deliver activated oxygen into the phagocytic vacuole.
Purification Method
Affinity purification
Gene ID
1536
RRID
AB_2862743
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using NOX2/gp91phox Rabbit mAb (CAB19701) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1min.
Western blot analysis of various lysates using NOX2/gp91phox Rabbit mAb (CAB19701) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 3min.
