The OAT Antibody (CAB6235) is a high-quality antibody developed for reliable detection and analysis of target proteins. This gene encodes the mitochondrial enzyme ornithine aminotransferase, which is a key enzyme in the pathway that converts arginine and ornithine into the major excitatory and inhibitory neurotransmitters glutamate and GABA. Mutations that result in a deficiency of this enzyme cause the autosomal recessive eye disease Gyrate Atrophy. Alternatively spliced transcript variants encoding different isoforms have been described. Related pseudogenes have been defined on the X chromosome. RRID AB_2766843 Gene ID 4942 Swiss Prot Synonym OKT; GACR; HOGA; OATASE; OAT
This antibody is validated for use in WB, IHC-P, IP, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
OAT Antibody
SKU:
CAB6235
Size:
100μL, 20μL
Reactivity:
Human, Mouse, Rat
Clone Number:
-
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBIHC-PIPELISA
Recommended Dilution:
WB
1:500 - 1:2000
IHC-P
1:50 - 1:200
IP
0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
OKT, GACR, HOGA, OATASE, OAT
Positive Sample:
Hep G2, 293T, Mouse liver, Mouse kidney, Rat liver
Cellular Localization:
Mitochondrion Matrix.
Calculated MW:
49kDa
Observed MW:
49kDa
This gene encodes the mitochondrial enzyme ornithine aminotransferase, which is a key enzyme in the pathway that converts arginine and ornithine into the major excitatory and inhibitory neurotransmitters glutamate and GABA. Mutations that result in a deficiency of this enzyme cause the autosomal recessive eye disease Gyrate Atrophy. Alternatively spliced transcript variants encoding different isoforms have been described. Related pseudogenes have been defined on the X chromosome. RRID AB_2766843 Gene ID 4942 Swiss Prot Synonym OKT; GACR; HOGA; OATASE; OAT
Purification Method:
Affinity purification
Gene ID:
4942
RRID:
AB_2766843
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using OAT Rabbit pAb (CAB6235) at 1:2000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Negative control (NC): RT4. Exposure time: 30s.
Immunohistochemistry analysis of paraffin-embedded Rat kidney using OAT Rabbit pAb (CAB6235) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunoprecipitation analysis of 200 μg extracts of MCF-7 cells, using 3 μg OAT antibody (CAB6235). Western blot was performed from the immunoprecipitate using OAT antibody (CAB6235) at a dilution of 1:1000.
