The OSR1 Antibody (CAB18272) is a high-quality antibody developed for reliable detection and analysis of target proteins. This high-quality antibody, produced in rabbits, exhibits strong reactivity with human samples and is validated for use in Western blot applications. It specifically binds to the OSR1 protein, allowing for precise detection and analysis in a variety of cell types.OSR1, also known as odd-skipped related transcription factor 1, plays a crucial role in embryonic development and kidney function.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
OSR1 Antibody
SKU:
CAB18272
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
ODD, OSR1
Positive Sample:
NCI-H460, Cal-27, A-549, 22Rv1
Cellular Localization:
Cytosol, Nucleus.
Calculated MW:
30kDa
Observed MW:
30kDa
Enables sequence-specific double-stranded DNA binding activity. Involved in negative regulation of ion transmembrane transporter activity; positive regulation of gastrulation; and pronephros development. Located in nucleus.
Purification Method
Affinity purification
Gene ID
130497
RRID
AB_2862046
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using OSR1 Rabbit pAb (CAB18272) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 180s.
Immunohistochemistry analysis of paraffin-embedded Rat kidney using OSR1 Rabbit pAb (CAB18272) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Mouse kidney using OSR1 Rabbit pAb (CAB18272) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
