The PAR4 Antibody (CAB3063) is a high-quality antibody developed for reliable detection and analysis of target proteins. PAWR is involved in apoptosis, cell cycle regulation, and tumor suppression, making it a key player in cancer research and other cellular processes.This antibody, raised in rabbits, exhibits high specificity and reactivity with human samples, making it ideal for a variety of research applications. It has been validated for use in Western blotting, immunofluorescence, and immunohistochemistry, allowing for the detection and analysis of PAWR expression in different cell types and tissues.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
PAR4 Antibody
SKU:
CAB3063
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
PAR4, Par-4
Positive Sample:
BT-474, HT-29, SKOV3, SW480, HepG2
Cellular Localization:
Cytoplasm, Nucleus.
Calculated MW:
37kDa
Observed MW:
43kDa
This gene encodes a tumor suppressor protein that selectively induces apoptosis in cancer cells through intracellular and extracellular mechanisms. The intracellular mechanism involves the inhibition of pro-survival pathways and the activation of Fas-mediated apoptosis, while the extracellular mechanism involves the binding of a secreted form of this protein to glucose regulated protein 78 (GRP78) on the cell surface, which leads to activation of the extrinsic apoptotic pathway. This gene is located on the unstable human chromosomal 12q21 region and is often deleted or mutated different tumors. The encoded protein also plays an important role in the progression of age-related diseases.
Purification Method
Affinity purification
Gene ID
5074
RRID
AB_2764866
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using PAR4 Rabbit pAb (CAB3063) at 1:400 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
