The PDK1/PDHK1 Monoclonal Antibody (CAB24749) is a high-quality antibody developed for reliable detection and analysis of target proteins. Pyruvate dehydrogenase (PDH) is a mitochondrial multienzyme complex that catalyzes the oxidative decarboxylation of pyruvate and is one of the major enzymes responsible for the regulation of homeostasis of carbohydrate fuels in mammals. The enzymatic activity is regulated by a phosphorylation/dephosphorylation cycle. Phosphorylation of PDH by a specific pyruvate dehydrogenase kinase (PDK) results in inactivation. Multiple alternatively spliced transcript variants have been found for this gene. RRID Gene ID 5163 Swiss Prot Synonym PDK1; pyruvate dehydrogenase kinase 1; PDK1/PDHK1
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
PDK1/PDHK1 Monoclonal Antibody
SKU:
CAB24749
Size:
100μL
Reactivity:
Human, Mouse
Clone Number:
ARC1355
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:1000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
PDK1, pyruvate dehydrogenase kinase 1, PDK1/PDHK1
Positive Sample:
HeLa, Mouse brain
Cellular Localization:
Mitochondrion Matrix.
Calculated MW:
49kDa
Observed MW:
49kDa
Pyruvate dehydrogenase (PDH) is a mitochondrial multienzyme complex that catalyzes the oxidative decarboxylation of pyruvate and is one of the major enzymes responsible for the regulation of homeostasis of carbohydrate fuels in mammals. The enzymatic activity is regulated by a phosphorylation/dephosphorylation cycle. Phosphorylation of PDH by a specific pyruvate dehydrogenase kinase (PDK) results in inactivation. Multiple alternatively spliced transcript variants have been found for this gene. RRID Gene ID 5163 Swiss Prot Synonym PDK1; pyruvate dehydrogenase kinase 1; PDK1/PDHK1
Purification Method:
Affinity purification
Gene ID:
5163
RRID:
-
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using PDK1/PDHK1 Rabbit mAb (CAB24749) at 1:1000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
